Fig 1: mRNA levels for Chrm1 - Chrm5, respectively encoding the M1-M5 receptors, quantified via qRT-PCR in BMVECs (A,C) and bEnd.3 cells (B,D). Data are normalized against two different reference genes, Gapdh (A,B) and Actb (C,D), and are plotted as mean ± SD (N = 5), from three different cell batches. One-way ANOVA analysis indicated: (A) F(32) = 80, p < 0.001; (B) F(25) = 56, p < 0.001; (C) F(25) = 58, p < 0.001; (D) F(14) = 121, p < 0.001. Statistical pairwise significance is indicated with asterisks (*0.01 < p < 0.05; **0.001 < p < 0.01; ***p < 0.001) followed by the compared variant.